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Lamin B1 (DGR16498) Rabbit mAb
db16165
Datasheet
- Background This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
- Immunogen Recombinant protein of human Lamin B1
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Gene ID
4001
16906
116685
-
Swiss Prot
P20700
P14733
P70615
- Synonyms LMN; ADLD; LMN2; LMNB; MCPH26
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
-
Recommended dilution
WB: 1:1000
IHC-P: 1:200-1:2000
ICC/IF: 1:500-1:1000
FC: 1:200-1:500
IP: 1:20-1:50 - Calculated MW 66 kDa
- Observed MW 70 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR16498
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of 3T3 cells labelling Lamin B1 with db16165.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16165 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Immunofluorescence analysis of HeLa cells labelling Lamin B1 with db16165.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16165 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF647(db10006, shown in red). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
