Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb - diagbio | 戴格生物

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Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb

db6251

Product Name : Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb

Cat.No.: db6251

Synonyms : H2AX; H2AFX; H2a/x; HIST5-2AX; Histone H2AX

Application : WB, ICC/IF

Reactivity : Human, Mouse

Host species : Mouse

Background Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X
Gene ID 3014
Swiss Prot P16104
Synonyms H2AX; H2AFX; H2a/x; HIST5-2AX; Histone H2AX
Reactivity Human, Mouse
Application WB, ICC/IF
Recommended dilution WB: 1:500-1:1000
ICC/IF: 1:50-1:200
Calculated MW 15 kDa
Observed MW 15 kDa
Host species Mouse
Clonality Monoclonal
Clonality No. 7G9-H4
Isotype IgG1
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.

Western blot analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyureatreated 3T3 lysates using Phospho-Histone H2A.X (Ser139) antibody.
Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (7G9) in 3T3 or Hydroxyureatreated 3T3 using Phospho-Histone H2A.X (Ser139) antibody.
Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (7G9) in A549(upper, untreated or Hydroxyureatreated) and Hela(lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.

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