His Tag单克隆抗体(DGR14407) | 高特异性&高亲和力

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Recombinant DGRmAb^®

His Tag (DGR14407) Rabbit mAb (PBS Only)

db11052-PBS

Datasheet

Product Name : His Tag (DGR14407) Rabbit mAb (PBS Only)

Cat.No.: db11052-PBS

Application：

Reactivity : Transfected

Host species : Rabbit

Un-conjugated PBS Only

Background Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Immunogen A synthetic peptide of His tag
Reactivity Transfected
Application WB, IHC-P, ICC/IF, FC, IP
Calculated MW 1 kDa
Observed MW According to the fused protein
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR14407
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Concentration 1 mg/mL
Formulation PBS Only
Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.

Western blot detection of His Tag in Multi-tag Protein，Multi-tag Protein in 293，Multi-tag Protein in HeLa cell lysates using His Tag antibody(1:1000 diluted).

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