Acetyl Coenzyme A carboxylase alpha单克隆抗体(DGR33624) | 高特异性&高亲和力

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Recombinant DGRmAb^®

Acetyl Coenzyme A carboxylase alpha (DGR33624) Rabbit mAb (PBS Only)

db11159-PBS

Datasheet

Product Name : Acetyl Coenzyme A carboxylase alpha (DGR33624) Rabbit mAb (PBS Only)

Cat.No.: db11159-PBS

Synonyms : ACC; ACAC; ACC1; ACCA; ACACAD

Application：

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Un-conjugated PBS Only

Background Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Immunogen Recombinant protein of human Acetyl Coenzyme A carboxylase alpha
Gene ID 31
107476
60581
Swiss Prot Q13085
Q5SWU9
P11497
Synonyms ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity Human,Mouse,Rat
Application WB, IHC-P, ICC/IF, FC, IP
Calculated MW 277 kDa
Observed MW 277 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR33624
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Concentration 1 mg/mL
Formulation PBS Only
Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells labelling Acetyl Coenzyme A carboxylase alpha with db11159.

The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11159 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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