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Hsp60 (DGR15603) Rabbit mAb (PBS Only)
db11164-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
- Immunogen Recombinant protein of human Hsp60
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Gene ID
3329
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Swiss Prot
P10809
- Synonyms HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
- Calculated MW 61 kDa
- Observed MW 60 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR15603
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of HeLa cells labelling Hsp60 with db11164.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11164 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
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For research use only. Not intended for diagnostic.
