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alpha Tubulin (DGR13966) Rabbit mAb (PBS Only)
db11363-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
- Immunogen A synthetic peptide of human alpha Tubulin
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Gene ID
7277
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Swiss Prot
P68366
- Synonyms ALS22; TUBA1; H2-ALPHA
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
- Calculated MW 50 kDa
- Observed MW 50 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13966
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa, HepG2, K562 cells and Rat lung tissue using db11363 at 1:5000. -
Western blot analysis of extracts from 3T3, RAW264.7, PC-12 cells and Mouse lung tissue using db11363 at 1:5000. -
Western blot analysis of extracts from CHO-K1 cells and Rat brain tissue using db11363 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling alpha Tubulin with db11363.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11363 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 2598
- 100μg 7858
- 货期：现货
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For research use only. Not intended for diagnostic.
