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GAPDH (DGR11217) Rabbit mAb (FITC)
db11729-FITC
Datasheet
- Background This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Alternative splicing results in multiple transcript variants. Many pseudogenes similar to this locus are found throughout the mouse genome. [provided by RefSeq, Jan 2014]
- Immunogen Recombinant protein of human GAPDH
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Gene ID
14433
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Swiss Prot
P16858
- Synonyms G3PD; GAPD; HEL-S-162eP
- Reactivity Human,Mouse,Rat,Monkey,Rabbit,Chicken,Zebrafish,Rabbit,Xenopus tropicalis,Chinese hamster,E. Escherichia coli
- Application ICC/IF, FC
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Recommended dilution
ICC/IF: 1:100-1:200
FC: 1:100 - Calculated MW 36 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11217
- Isotype IgG
- Purity Affinity Purification
- Conjugation FITC
- Concentration 1 mg/mL
- Storage/Stability Store at -20°C. Avoid exposure to light. Supplied in PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of HeLa cells labelling GAPDH with db11729-FITC.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11729-FITC (1:100，shown in green) at room temprature for 1h. Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μL 1198
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
