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Acetyl-Histone H3 (Lys27) (DGR14746) Rabbit mAb (PBS Only)
db11759-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
- Immunogen A synthetic acetyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
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Gene ID
8356
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Swiss Prot
P68431
- Synonyms H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
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Recommended dilution
WB: 1:5000-1:50000
IHC-P: 1:1000-1:10000
ICC/IF: 1:500-1：5000
FC: 1:200-1:1000 - Calculated MW 15 kDa
- Observed MW 17 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14746
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/ml
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot detection of Histone H3 (acetyl-Lys27) in Hela,A549,HL-60 cell lysates using Histone H3 (acetyl-Lys27) antibody(1:1000 diluted). -
Western blot analysis of extracts from C6 cells using db11759
订购信息
- Package Price (RMB)
- 100μg 4498
- 货期：现货
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For research use only. Not intended for diagnostic.
