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Hsp70 (DGR16508) Rabbit mAb (PBS Only)
db13325-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
- Immunogen Recombinant protein of mouse Hsp70
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Gene ID
3303
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Swiss Prot
P0DMV8
- Synonyms HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
- Calculated MW 70 kDa
- Observed MW 70 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR16508
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa cells using db13325 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human tonsil using db13325 antibody. -
Immunofluorescence analysis of HeLa cells labelling Hsp70 with db13325.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db13325 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 4498
- 货期：现货
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For research use only. Not intended for diagnostic.
