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Acetyl Coenzyme A Carboxylase (DGR20960) Rabbit mAb (PBS Only)
db14523-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human Acetyl Coenzyme A Carboxylase
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Gene ID
31
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Swiss Prot
O00763
- Synonyms ACC; ACAC; ACC1; ACCA; ACACAD
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC
- Calculated MW 277 kDa
- Observed MW 277 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR20960
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot detection of Acetyl Coenzyme A Carboxylase in Hela,HCT116 cell lysates using Acetyl Coenzyme A Carboxylase antibody(1:1000 diluted). -
Immunofluorescence analysis of HeLa cells labelling Acetyl Coenzyme A Carboxylase with db14523.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14523 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
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For research use only. Not intended for diagnostic.
