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FACL4 (DGR33206) Rabbit mAb (PBS Only)
db14837-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016]
- Immunogen Recombinant protein of human FACL4
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Gene ID
2182
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Swiss Prot
O60488
- Synonyms ACSL4; ACS4; FACL4; LACS4; MRX63; MRX68
- Reactivity Human,Mouse
- Application WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000
IHC-P: 1:200-1:500
ICC/IF: 1:50
FC: 1:100
IP: 1:20-1:50 - Calculated MW 79 kDa
- Observed MW 79 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR33206
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/ml
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot detection of FACL4 in Hela,A549 using db14837(1:1000 diluted) -
Immunofluorescence analysis of HeLa cells labelling FACL4 with db14837.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14837 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
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For research use only. Not intended for diagnostic.
