您当前的位置：
Chk1 (DGR12192) Rabbit mAb (PBS Only)
db14895-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]
- Immunogen A synthetic peptide of human Chk1
-
Gene ID
1111
-
Swiss Prot
O14757
- Synonyms CHK1
- Reactivity Human,Mouse,Rat
- Application WB, ICC/IF, FC, IP
- Calculated MW 54 kDa
- Observed MW 54 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12192
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
![]()
-
Western blot detection of Chk1 in K562,3T3,Hela cell lysates using Chk1 antibody(1:1000 diluted). -
Immunofluorescence analysis of HeLa cells labelling Chk1 with db14895.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14895 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
