SOX17单克隆抗体(DGR14493) | 高特异性&高亲和力

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Recombinant DGRmAb^®

SOX17 (DGR14493) Rabbit mAb

db15286

Datasheet

Product Name : SOX17 (DGR14493) Rabbit mAb

Cat.No.: db15286

Synonyms : VUR3

Application : WB, IHC-P, ICC/IF, IP

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Background This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. [provided by RefSeq, Jul 2008]
Immunogen A synthetic peptide of human SOX17
Gene ID 64321
Swiss Prot Q9H6I2
Synonyms VUR3
Reactivity Human, Mouse, Rat
Application WB, IHC-P, ICC/IF, IP
Recommended dilution WB: 1:1000
IHC-P: 1:200-1:2000
ICC/IF: 1:200-1:2000
IP: 1:20-1:50
Calculated MW 44 kDa
Observed MW 55 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR14493
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells labelling SOX17 with db15286.

The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15286 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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