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53BP1 (DGR14147) Rabbit mAb (PBS Only)
db15410-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
- Immunogen A synthetic peptide of human 53BP1
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Gene ID
7158
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Swiss Prot
Q12888
- Synonyms TP53; p202; 53BP1; TDRD30; p53BP1
- Reactivity Human, Mouse, Rat
- Application WB, IHC, ICC/IF, FC
- Calculated MW 214 kDa
- Observed MW 450 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14147
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa cells using db15410 at 1:1000. -
Immunofluorescence analysis of HepG2 cells labelling 53BP1 with db15410.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15410 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
