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Calpain 1 (DGR14045) Rabbit mAb (PBS Only)
db15448-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
- Immunogen A synthetic peptide of human Calpain 1
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Gene ID
823
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Swiss Prot
P07384
- Synonyms CANP; muCL; CANP1; SPG76; CANPL1; muCANP
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
- Calculated MW 82 kDa
- Observed MW 82 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14045
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Immunofluorescence analysis of HeLa cells labelling Calpain 1 with db15448.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15448 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 4498
- 货期：现货
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For research use only. Not intended for diagnostic.
