RHEB (DGR13216) Rabbit mAb (PBS Only)
Product Name :
RHEB (DGR13216) Rabbit mAb (PBS Only)
Cat.No.:
db15626-PBS
Synonyms :
RHEB2
Reactivity :
Human
Host species :
Rabbit
Conjugation/Formulation:PBS Only
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Background
This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]
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Immunogen
A synthetic peptide of human RHEB
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Gene ID
6009
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Swiss Prot
Q15382
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Synonyms
RHEB2
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Reactivity
Human
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Application
WB, IHC-P, ICC/IF, FC
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Calculated MW
21 kDa
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Observed MW
21 kDa
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Host species
Rabbit
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Clonality
Monoclonal
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Clonality No.
DGR13216
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Isotype
IgG
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Purity
Affinity Purification
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Conjugation
Un-conjugated
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Concentration
1 mg/mL
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Formulation
PBS Only
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Storage/Stability
Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
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Western blot analysis of extracts from SH-SY5Y cells using
db15626 at 1:1000.
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Immunofluorescence analysis of Neuro-2a cells labelling RHEB with
db15626.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with
db15626 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488
db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
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Package
Price (RMB)
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100μg
5798
- 货期:现货
For research use only. Not intended for diagnostic.