产品中心 Products Center
RHEB (DGR13216) Rabbit mAb (PBS Only)
db15626-PBS
Datasheet

Product Name : RHEB (DGR13216) Rabbit mAb (PBS Only)

Cat.No.: db15626-PBS

Synonyms : RHEB2

Application:

Reactivity : Human

Host species : Rabbit

Conjugation/Formulation:PBS Only

  • Background This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]
  • Immunogen A synthetic peptide of human RHEB
  • Gene ID 6009
  • Swiss Prot Q15382
  • Synonyms RHEB2
  • Reactivity Human
  • Application WB, IHC-P, ICC/IF, FC
  • Calculated MW 21 kDa
  • Observed MW 21 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR13216
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/mL
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
  • Western blot analysis of extracts from SH-SY5Y cells using db15626 at 1:1000.
  • Immunofluorescence analysis of Neuro-2a cells labelling RHEB with db15626.

    The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15626 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 100μg 5798
  • 货期:现货
For research use only. Not intended for diagnostic.