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MEK1 (DGR11737) Rabbit mAb (PBS Only)
db15920-PBS
Datasheet

Product Name : MEK1 (DGR11737) Rabbit mAb (PBS Only)

Cat.No.: db15920-PBS

Synonyms : CFC3; MEK1; MKK1; MAPKK1; PRKMK1

Application:

Reactivity : Human

Host species : Rabbit

Conjugation/Formulation:PBS Only

  • Background The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
  • Immunogen A synthetic peptide of human MEK1
  • Gene ID 5604
  • Swiss Prot Q02750
  • Synonyms CFC3; MEK1; MKK1; MAPKK1; PRKMK1
  • Reactivity Human
  • Application WB, IHC-P, ICC/IF, FC, IP
  • Calculated MW 43 kDa
  • Observed MW 43 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR11737
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/mL
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
  • Western blot analysis of extracts from Jurkat cells using db15920 at 1:1000.
  • Immunofluorescence analysis of HeLa cells labelling MEK1 with db15920.

    The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15920 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 100μg 5798
  • 货期:现货
For research use only. Not intended for diagnostic.