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PDCD4 (DGR11522) Rabbit mAb
db15975
Datasheet
- Background This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
- Immunogen A synthetic peptide of human PDCD4
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Gene ID
27250
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Swiss Prot
Q53EL6
- Synonyms H731
- Reactivity Human,Mouse
- Application WB, IHC-P, ICC/IF
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:500-1:2000
ICC/IF: 1:200-1:500 - Calculated MW 52 kDa
- Observed MW 52 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11522
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from Jurkat cells using db15975 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling PDCD4 with db15975.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15975(1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
