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DDX17 (DGR36086) Rabbit mAb (PBS Only)
db16321-PBS
Datasheet

Product Name : DDX17 (DGR36086) Rabbit mAb (PBS Only)

Cat.No.: db16321-PBS

Synonyms : P72; RH70

Application:

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Conjugation/Formulation:PBS Only

  • Background DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon. [provided by RefSeq, Apr 2011]
  • Immunogen A synthetic peptide of human DDX17
  • Gene ID 10521
  • Swiss Prot Q92841
  • Synonyms P72; RH70
  • Reactivity Human, Mouse, Rat
  • Application WB, IHC-P, ICC/IF, FC
  • Calculated MW 80 kDa
  • Observed MW 72, 82 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR36086
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/mL
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
  • Immunofluorescence analysis of HeLa cells labelling DDX17 with db16321.

    The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16321 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 10μg 2598
  • 100μg 7858
  • 货期:现货
For research use only. Not intended for diagnostic.