Product Name :
EIF2S1 (2E4F5) Mouse mAb
Cat.No.:
db6265
Synonyms :
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Reactivity :
Human, Mouse, Rat
Host species :
Mouse
Conjugation/Formulation:Un-conjugated
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Background
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
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Immunogen
Purified recombinant human eIF2α protein fragments expressed in E.coli
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Gene ID
1965
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Swiss Prot
P05198
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Synonyms
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
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Reactivity
Human, Mouse, Rat
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Application
WB, ICC/IF, IP
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Recommended dilution
WB: 1:500-1:1000
ICC/IF: 1:50-1:200
IP: 1:20
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Calculated MW
36 kDa
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Observed MW
36 kDa
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Host species
Mouse
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Clonality
Monoclonal
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Clonality No.
2E4-F5-E7
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Isotype
IgG2b
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Purity
Affinity Purification
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Conjugation
Un-conjugated
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Storage/Stability
Store at -20°C. Supplied in PBS, 50% Glycerol(pH 7.3), 0.02% sodium azide and 0.5% BSA . Stable for 12 months from date of receipt.
×
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Western blot analysis of eIF2α in K562, COS7, 3T3, C2C12, MCF-7, C6 and Hela lysates using eIF2α antibody.
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Immunofluorescence analysis of eIF2A (2E4F5) in Hela using eIF2α antibody.
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Immunoprecipitation analysis of eIF2A (2E4F5) in Hela lysates using eIF2α antibody.
订购信息
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Package
Price (RMB)
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50μL
1398
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100μL
2398
- 货期:现货
For research use only. Not intended for diagnostic.