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Conjugation/Formulation：Un-conjugated
- Background The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]
- Immunogen A synthetic peptide of human Lamin A/C
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Gene ID
4000
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Swiss Prot
P02545
- Synonyms FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
- Reactivity Human, Mouse, Rat
- Application WB, IHC-P, ICC/IF
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Recommended dilution
WB: 1:2000-1:20000
IHC-P: 1:100-1:200
ICC/IF: 1:200-1:500 - Calculated MW 74 kDa
- Observed MW 74,63 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR31266
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from U251, LNCaP, A549 cells using db11008 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human breast cancer using db11008 antibody. -
Western blot analysis of extracts from C2C12 cells using db11008 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Lamin A/C with db11008.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11008 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
