Lamin A,Lamin C单克隆抗体(DGR31266) | 高特异性&高亲和力

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Recombinant DGRmAb^®

Lamin A/C (DGR31266) Rabbit mAb (PBS Only)

db11008-PBS

Product Name : Lamin A/C (DGR31266) Rabbit mAb (PBS Only)

Cat.No.: db11008-PBS

Synonyms : FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B

Application：

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Un-conjugated AF488 AF647 PBS Only

Background The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]
Immunogen A synthetic peptide of human Lamin A/C
Gene ID 4000
Swiss Prot P02545
Synonyms FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity Human, Mouse, Rat
Application WB, IHC-P, ICC/IF
Calculated MW 74 kDa
Observed MW 74,63 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR31266
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Concentration 1 mg/mL
Formulation PBS Only
Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.

Western blot analysis of extracts from U251, LNCaP, A549 cells using db11008 at 1:1000.
Immunohistochemical analysis of paraffin-embedded human breast cancer using db11008 antibody.
Western blot analysis of extracts from C2C12 cells using db11008 at 1:1000.
Immunofluorescence analysis of HeLa cells labelling Lamin A/C with db11008.

The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11008 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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