Hsp60 (DGR15603) Rabbit mAb
Product Name :
Hsp60 (DGR15603) Rabbit mAb
Cat.No.:
db11164
Synonyms :
HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity :
Human, Mouse, Rat
Host species :
Rabbit
Conjugation/Formulation:Un-conjugated
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Background
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
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Immunogen
Recombinant protein of human Hsp60
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Gene ID
3329
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Swiss Prot
P10809
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Synonyms
HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
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Reactivity
Human,Mouse,Rat
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Application
WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000
IHC-P: 1:200-1:2000
ICC/IF: 1:100-1:200
FC: 1:200-1:500
IP: 1:20-1:50
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Calculated MW
61 kDa
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Observed MW
60 kDa
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Host species
Rabbit
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Clonality
Monoclonal
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Clonality No.
DGR15603
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Isotype
IgG
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Purity
Affinity Purification
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Conjugation
Un-conjugated
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Storage/Stability
Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
×
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Immunofluorescence analysis of HeLa cells labelling Hsp60 with db11164.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11164 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (
db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
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Package
Price (RMB)
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10μL
398
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20μL
698
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50μL
1398
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100μL
2398
- 货期:现货
For research use only. Not intended for diagnostic.