Amyloid Precursor Protein单克隆抗体(DGR11972) | 高特异性&高亲和力

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Recombinant DGRmAb^®

Amyloid Precursor Protein (DGR11972) Rabbit mAb

db12005

618特价

Datasheet

Product Name : Amyloid Precursor Protein (DGR11972) Rabbit mAb

Cat.No.: db12005

Synonyms : AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma

Application：

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Un-conjugated PBS Only

Background This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]
Immunogen A synthetic peptide of human Amyloid Precursor Protein
Gene ID 351
Swiss Prot P05067
Synonyms AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
Reactivity Human,Mouse,Rat
Application WB, IHC-P, ICC/IF, IP
Recommended dilution WB: 1:2000-1:20000
IHC-P: 1:200-1:500
ICC/IF: 1:100-1:500
IP: 1:20-1:50
Calculated MW 87 kDa
Observed MW 100 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR11972
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Western blot analysis of extracts from 3T3, HeLa cells and Rat brain tissue using db12005 at 1:1000.
Western blot analysis of extracts from Mouse brain tissue using db12005 at 1:1000.
Immunohistochemical analysis of paraffin-embedded human brain using db12005 antibody.
Immunofluorescent analysis of HeLa cells using db12005 antibody (green), and DAPI (blue).
Immunofluorescence analysis of HeLa cells labelling Amyloid Precursor Protein with db12005.

The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12005 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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