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Conjugation/Formulation：Un-conjugated
- Background This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
- Immunogen A synthetic peptide of human Cytochrome C
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Gene ID
54205
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Swiss Prot
P99999
- Synonyms CYC; HCS; THC4
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, IP
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:200-1:500
ICC/IF: 1:100
IP: 1:20-1:50 - Calculated MW 12 kDa
- Observed MW 14 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13732
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from Jurkat, C6 cells and Rat brain tissue using db12144 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human tonsil using db12144 antibody. -
Western blot analysis of extracts from Mouse brain tissue using db12144 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Cytochrome C with db12144.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12144 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
