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Cytochrome C (DGR13732) Rabbit mAb (PBS Only)
db12144-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
- Immunogen A synthetic peptide of human Cytochrome C
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Gene ID
54205
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Swiss Prot
P99999
- Synonyms CYC; HCS; THC4
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, IP
- Calculated MW 12 kDa
- Observed MW 14 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13732
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from Jurkat, C6 cells and Rat brain tissue using db12144 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human tonsil using db12144 antibody. -
Western blot analysis of extracts from Mouse brain tissue using db12144 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Cytochrome C with db12144.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12144 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
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For research use only. Not intended for diagnostic.
