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Conjugation/Formulation：Un-conjugated
- Background This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
- Immunogen A synthetic peptide of human PUMA
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Gene ID
27113
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Swiss Prot
Q9BXH1
- Synonyms JFY1; PUMA; JFY-1
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:100
ICC/IF: 1:200-1:500
FC: 1:20-1:100 - Calculated MW 21 kDa
- Observed MW 21 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14872
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from Jurkat, THP-1, C6, 3T3, HeLa cells using db12341 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human breast cancer using db12341 antibody. -
Immunofluorescent analysis of 293T cells using db12341 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling PUMA with db12341.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12341 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
