您当前的位置：
Conjugation/Formulation：Un-conjugated
- Background This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers. [provided by RefSeq, Jul 2016]
- Immunogen A synthetic peptide of human BMP4
-
Gene ID
652
-
Swiss Prot
P12644
- Synonyms ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC, IP
-
Recommended dilution
WB: 1:2000-1:20000
IHC-P: 1:100-1:200
ICC/IF: 1:200-1:500
FC: 1:100-1:500
IP: 1:10-1:100 - Calculated MW 47 kDa
- Observed MW 47 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR15418
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
×
![]()
-
Western blot analysis of extracts from K562, HeLa cells using db12985 at 1:1000. -
Western blot analysis of extracts from HepG2 cells using db12985 at 1:5000. -
Immunofluorescent analysis of HepG2 cells using db12985 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling BMP4 with db12985.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db12985 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
