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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human SMARCC1
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Gene ID
6599
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Swiss Prot
Q92922
- Synonyms Rsc8; SRG3; SWI3; BAF155; CRACC1
- Reactivity Human,Rat
- Application WB, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000-1:5000
ICC/IF: 1:100
FC: 1:10-1:100
IP: 1:10-1:100 - Calculated MW 123 kDa
- Observed MW 155 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14464
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, C6, HeLa cells using db14615 at 1:1000. -
Immunofluorescent analysis of HT-1080 cells using db14615 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling SMARCC1 with db14615.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14615 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
