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SMARCC1 (DGR14464) Rabbit mAb (PBS Only)
db14615-PBS
Datasheet

Product Name : SMARCC1 (DGR14464) Rabbit mAb (PBS Only)

Cat.No.: db14615-PBS

Synonyms : Rsc8; SRG3; SWI3; BAF155; CRACC1

Application:

Reactivity : Human, Rat

Host species : Rabbit

Conjugation/Formulation:PBS Only

  • Background The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq, Jul 2008]
  • Immunogen A synthetic peptide of human SMARCC1
  • Gene ID 6599
  • Swiss Prot Q92922
  • Synonyms Rsc8; SRG3; SWI3; BAF155; CRACC1
  • Reactivity Human,Rat
  • Application WB, ICC/IF, FC, IP
  • Calculated MW 123 kDa
  • Observed MW 155 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR14464
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/mL
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
  • Western blot analysis of extracts from K562, C6, HeLa cells using db14615 at 1:1000.
  • Immunofluorescent analysis of HT-1080 cells using db14615 antibody (green), and DAPI (blue).
  • Immunofluorescence analysis of HeLa cells labelling SMARCC1 with db14615.

    The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14615 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 10μg 1898
  • 100μg 5798
  • 货期:现货
For research use only. Not intended for diagnostic.