c-Myc单克隆抗体(DGR12778) | 高特异性&高亲和力

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Recombinant DGRmAb^®

c-Myc (DGR12778) Rabbit mAb

db14926

Datasheet

Product Name : c-Myc (DGR12778) Rabbit mAb

Cat.No.: db14926

Synonyms : MRTL; MYCC; c-Myc; bHLHe39

Application : WB, IHC-P, ICC/IF, FC, IP

Reactivity : Human,Mouse,Rat

Host species : Rabbit

Background This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
Immunogen A synthetic peptide of human c-Myc
Gene ID 4609
Swiss Prot P01106
Synonyms MRTL; MYCC; c-Myc; bHLHe39
Reactivity Human,Mouse,Rat
Application WB, IHC-P, ICC/IF, FC, IP
Recommended dilution WB: 1:1000
IHC-P: 1:50-1:500
ICC/IF: 1:20-1:200
FC: 1:50-1:100
IP: 1:20-1:50
Calculated MW 49 kDa
Observed MW 57-65 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR12778
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Western blot detection of c-Myc in HeLa cell lysates using c-Myc antibody(1:1000 diluted).
Immunofluorescent analysis of HeLa cells using db14926 antibody (green), and DAPI (blue).
Immunofluorescence analysis of HeLa cells labelling c-Myc with db14926.

The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14926 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.
Western blot analysis of c-Myc in K562, C6, 3T3, Hela lysates using c-Myc antibody(1:1000 diluted).

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