您当前的位置：
c-Myc (DGR12778) Rabbit mAb (PBS Only)
db14926-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
- Immunogen A synthetic peptide of human c-Myc
-
Gene ID
4609
-
Swiss Prot
P01106
- Synonyms MRTL; MYCC; c-Myc; bHLHe39
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
- Calculated MW 49 kDa
- Observed MW 57-65 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12778
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
![]()
-
Western blot detection of c-Myc in HeLa cell lysates using c-Myc antibody(1:1000 diluted). -
Immunofluorescent analysis of HeLa cells using db14926 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling c-Myc with db14926.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db14926 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Western blot analysis of c-Myc in K562, C6, 3T3, Hela lysates using c-Myc antibody(1:1000 diluted).
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
