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MEK1 (DGR11737) Rabbit mAb
db15920
Datasheet
- Background The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human MEK1
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Gene ID
5604
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Swiss Prot
Q02750
- Synonyms CFC3; MEK1; MKK1; MAPKK1; PRKMK1
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:100
ICC/IF: 1:50
FC: 1:20-1:50
IP: 1:20-1:50 - Calculated MW 43 kDa
- Observed MW 43 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11737
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from Jurkat cells using db15920 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling MEK1 with db15920.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db15920 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
