Cytokeratin 8单克隆抗体(DGR11203) | 高特异性&高亲和力

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Recombinant DGRmAb^®

Cytokeratin 8 (DGR11203) Rabbit mAb

db16057

Datasheet

Product Name : Cytokeratin 8 (DGR11203) Rabbit mAb

Cat.No.: db16057

Synonyms : K8; KO; CK8; CK-8; CYK8; K2C8; CARD2

Application : WB, IHC-P, ICC/IF, FC, IP

Reactivity : Human,Mouse,Rat

Host species : Rabbit

Background This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Immunogen A synthetic peptide of human Cytokeratin 8
Gene ID 3856
Swiss Prot P05787
Synonyms K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Reactivity Human,Mouse,Rat
Application WB, IHC-P, ICC/IF, FC, IP
Recommended dilution WB: 1:1000-1:5000
IHC-P: 1:100-1:200
ICC/IF: 1:100-1:500
FC: 1:20-1:50
IP: 1:20
Calculated MW 54 kDa
Observed MW 54 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR11203
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Western blot analysis of extracts from MCF-7 cells using db16057 at 1:5000.
Immunofluorescence analysis of HeLa cells labelling Cytokeratin 8 with db16057.

The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16057 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.
Immunofluorescence analysis of HeLa cells labelling Cytokeratin 8 with db16057.

The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16057 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF647 (db10006, shown in red). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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