您当前的位置：
Cytokeratin 8 (DGR11203) Rabbit mAb (PBS Only)
db16057-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
- Immunogen A synthetic peptide of human Cytokeratin 8
-
Gene ID
3856
-
Swiss Prot
P05787
- Synonyms K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
- Calculated MW 54 kDa
- Observed MW 54 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11203
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
![]()
-
Western blot analysis of extracts from MCF-7 cells using db16057 at 1:5000. -
Immunofluorescence analysis of HeLa cells labelling Cytokeratin 8 with db16057.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16057 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only. -
Immunofluorescence analysis of HeLa cells labelling Cytokeratin 8 with db16057.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db16057 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF647 db10006, shown in red). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 可询价
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
