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Conjugation/Formulation：Un-conjugated
- Background The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq, Mar 2010]
- Immunogen A synthetic peptide of human beta Tubulin
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Gene ID
347733
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Swiss Prot
Q9BVA1
- Synonyms CDCBM7; PMGYSA; bA506K6.1
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
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Recommended dilution
WB: 1:1000
IHC-P: 1:100-1:200
ICC/IF: 1:500-1:1000
FC: 1:100 - Calculated MW 50 kDa
- Observed MW 50 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR11132
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa, Jurkat, HepG2, K562, C6 cells and Rat brain, Rat lung tissues using db11073 at 1:5000. -
Western blot analysis of extracts from 3T3, RAW264.7, PC-12 and Mouse lung tissue using db11073 at 1:5000. -
Western blot analysis of extracts from Mouse brain tissue using db11073 at 1:5000. -
Immunofluorescence analysis of HeLa cells labelling beta Tubulin with db11073.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11073 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 100μL 998
- 500μL 3998
- 1mL 6998
- 货期：现货
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For research use only. Not intended for diagnostic.
