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PARP1 (DGR14196) Rabbit mAb (PBS Only)
db11121-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human PARP1
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Gene ID
142
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Swiss Prot
P09874
- Synonyms PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF
- Calculated MW 113 kDa
- Observed MW 116,89 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14196
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, 3T3, and HeLa cells using db11121 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human tonsil using db11121 antibody. -
Immunofluorescent analysis of HeLa cells using db11121 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of K562 cells labelling PARP1 with db11121.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11121 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 1498
- 100μg 4498
- 货期：现货
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For research use only. Not intended for diagnostic.
