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alpha B Crystallin (DGR12950) Rabbit mAb (PBS Only)
db11173-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
- Immunogen A synthetic peptide of human Alpha B Crystallin
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Gene ID
1410
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Swiss Prot
P02511
- Synonyms MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
- Reactivity Human,Rat
- Application WB, IHC-P, ICC/IF, IP
- Calculated MW 20 kDa
- Observed MW 22 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12950
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from C6, HeLa cells using db11173 at 1:1000. -
Immunofluorescent analysis of HeLa cells using db11173 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling alpha B Crystallin with db11173.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11173 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 2598
- 100μg 7858
- 货期：现货
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For research use only. Not intended for diagnostic.
