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Conjugation/Formulation：Un-conjugated
- Background Predicted to enable actin filament binding activity. Predicted to be involved in Arp2/3 complex-mediated actin nucleation and cell migration. Located in extracellular exosome and focal adhesion. [provided by Alliance of Genome Resources, Apr 2022]
- Immunogen A synthetic peptide of human ARPC5L
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Gene ID
81873
74192
296710
-
Swiss Prot
Q9BPX5
Q9D898
A1L108
- Synonyms ARC16-2
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC
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Recommended dilution
WB: 1:1000-1:5000
IHC-P: 1:200-1:500
ICC/IF: 1:200-1:500
FC: 1:10-1:100 - Calculated MW 17 kDa
- Observed MW 17 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR35538
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from U-87 MG cells using db11204 at 1:1000. -
Immunofluorescence analysis of MCF-7 cells labelling ARPC5L with db11204.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11204 (1:200) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
