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Conjugation/Formulation：Un-conjugated
- Background This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human eEF1A1
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Gene ID
1915
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Swiss Prot
P68104
- Synonyms CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
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Recommended dilution
WB: 1:2000-1:20000
IHC-P: 1:100
ICC/IF: 1:100
FC: 1:50
IP: 1:10-1:100 - Calculated MW 50 kDa
- Observed MW 50 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR16028
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, C6, 3T3, HeLa cells using db11229 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human tonsil using db11229 antibody. -
Immunofluorescence analysis of MCF-7 cells labelling eEF1A1 with db11229.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11229 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
