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Conjugation/Formulation：Un-conjugated
- Background This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
- Immunogen Recombinant protein of human DGCR8
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Gene ID
54487
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Swiss Prot
Q8WYQ5
- Synonyms Gy1; pasha; DGCRK6; C22orf12
- Reactivity Human,Mouse,Rat
- Application WB, ICC/IF, FC, IP
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Recommended dilution
WB: 1:1000
ICC/IF: 1:500-1:1000
FC: 1:200-1:1000
IP: 1:50 - Calculated MW 86 kDa
- Observed MW 120 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR19704
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, C6, 3T3 cells using db11246 at 1:1000. -
Immunofluorescent analysis of K562 cells using db11246 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling DGCR8 with db11246.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11246 (1:500) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
