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Conjugation/Formulation：Un-conjugated
- Background Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq, Jun 2010]
- Immunogen A synthetic peptide of human Dynamin-2
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Gene ID
1785
13430
25751
-
Swiss Prot
P50570
P39054
P39052
- Synonyms DYN2; CMT2M; DYNII; LCCS5; CMTDI1; CMTDIB; DI-CMTB
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF
-
Recommended dilution
WB: 1:1000
IHC-P: 1:50-1:100
ICC/IF: 1:50 - Calculated MW 98 kDa
- Observed MW 98 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR35836
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Storage/Stability Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa cells using db11304 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Dynamin-2 with db11304.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11304 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μL 398
- 20μL 698
- 50μL 1398
- 100μL 2398
- 货期：现货
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For research use only. Not intended for diagnostic.
