HEXIM1单克隆抗体(DGR33700) | 高特异性&高亲和力

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Recombinant DGRmAb^®

HEXIM1 (DGR33700) Rabbit mAb (PBS Only)

db11334-PBS

Datasheet

Product Name : HEXIM1 (DGR33700) Rabbit mAb (PBS Only)

Cat.No.: db11334-PBS

Synonyms : CLP1; EDG1; HIS1; MAQ1

Application：

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Un-conjugated PBS Only

Background Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]
Immunogen A synthetic peptide of human HEXIM1
Gene ID 10614
192231
498008
Swiss Prot O94992
Q8R409
Q5M9G1
Synonyms CLP1; EDG1; HIS1; MAQ1
Reactivity Human,Mouse,Rat
Application WB, IHC-P, ICC/IF, FC, IP
Recommended dilution WB: 1:1000
IHC-P: 1:200-1:2000
ICC/IF: 1:50
FC: 1:200-1:500
IP: 1:20-1:50
Calculated MW 41 kDa
Observed MW 60 kDa
Host species Rabbit
Clonality Monoclonal
Clonality No. DGR33700
Isotype IgG
Purity Affinity Purification
Conjugation Un-conjugated
Concentration 1 mg/ml
Formulation PBS Only
Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.

Western blot analysis of extracts from LNCaP cells using db11334 at 1:1000.
Immunofluorescence analysis of MCF-7 cells labelling HEXIM1 with db11334.

The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11334 (1:50) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

Control: Secondary antibody only.

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