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A RAF (DGR31540) Rabbit mAb (PBS Only)
db11447-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]
- Immunogen Recombinant protein of human A RAF
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Gene ID
369
-
Swiss Prot
P10398
- Synonyms PKS2; A-RAF; ARAF1; RAFA1
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC
- Calculated MW 68 kDa
- Observed MW 68 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR31540
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot detection of A RAF in Hela,Ramos cell lysates using A RAF antibody(1:1000 diluted). -
Western blot analysis of extracts from SCC-9,U2OS cells using db11447 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human lung cancer using db11447 antibody. -
Immunofluorescence analysis of HeLa cells labelling A RAF with db11447.
The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11447 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 5798
- 货期：现货
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For research use only. Not intended for diagnostic.
