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PCNA (DGR14426) Rabbit mAb (PBS Only)
db11523-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
- Immunogen A synthetic peptide of human PCNA
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Gene ID
5111
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Swiss Prot
P12004
- Synonyms ATLD2
- Reactivity Human,Mouse,Rat
- Application WB, IHC-P, ICC/IF, FC, IP
- Calculated MW 29 kDa
- Observed MW 36 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14426
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from K562, C6 cells using db11523 at 1:1000. -
Western blot analysis of extracts from HeLa cells using db11523 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling PCNA with db11523.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11523 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 100μg 7858
- 货期：现货
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For research use only. Not intended for diagnostic.
