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Occludin (DGR14439) Rabbit mAb (PBS Only)
db11580-PBS
Datasheet

Product Name : Occludin (DGR14439) Rabbit mAb (PBS Only)

Cat.No.: db11580-PBS

Synonyms : BLCPMG; PTORCH1; PPP1R115

Application:

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Conjugation/Formulation:PBS Only

  • Background This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
  • Immunogen Recombinant protein of human Occludin
  • Gene ID 100506658
    18260
    83497
  • Swiss Prot Q16625
    Q61146
    Q6P6T5
  • Synonyms BLCPMG; PTORCH1; PPP1R115
  • Reactivity Human,Mouse,Rat
  • Application WB, IHC-P, ICC/IF, FC, IP
  • Calculated MW 59 kDa
  • Observed MW 65 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR14439
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/mL
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
  • Western blot analysis of extracts from Mouse brain tissue using db11580 at 1:1000.
  • Immunofluorescence analysis of HT-29 cells labelling Occludin with db11580.

    The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11580 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 10μg 1498
  • 100μg 4498
  • 货期:现货
For research use only. Not intended for diagnostic.