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Vimentin (DGR12122) Rabbit mAb (PBS Only)
db11589-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
- Immunogen A synthetic peptide of human Vimentin
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Gene ID
7431
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Swiss Prot
P08670
- Synonyms VIM
- Reactivity Human, Mouse, Rat
- Application WB, IHC, ICC/IF, FC
- Calculated MW 54 kDa
- Observed MW 54 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR12122
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from C6, CHO-K1, HeLa cells using db11589 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human tonsil using db11589 antibody. -
Immunofluorescent analysis of HeLa cells using db11589 antibody (green), and DAPI (blue). -
Western blot analysis of extracts from 3T3 cells using db11589 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Vimentin with db11589.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11589 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 2598
- 100μg 7858
- 货期：现货
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For research use only. Not intended for diagnostic.
