您当前的位置：
MBD2 (DGR14399) Rabbit mAb (PBS Only)
db11623-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
- Immunogen A synthetic peptide of human MBD2
-
Gene ID
8932
-
Swiss Prot
Q9UBB5
- Synonyms DMTase; NY-CO-41
- Reactivity Human, Mouse, Rat
- Application WB, IHC, ICC/IF, FC, IP
- Calculated MW 43 kDa
- Observed MW 43 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR14399
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
![]()
-
Western blot analysis of extracts from C6, 3T3, HeLa cells and Rat brain tissue using db11623 at 1:1000. -
Immunohistochemical analysis of paraffin-embedded human tonsil using db11623 antibody. -
Immunofluorescent analysis of HL-60 cells using db11623 antibody (green), and DAPI (blue). -
Immunofluorescence analysis of HeLa cells labelling MBD2 with db11623.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11623 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 1898
- 100μg 5798
- 货期：现货
相关产品
For research use only. Not intended for diagnostic.
