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Recombinant DGRmAb®
ERK1/2 (DGR11869) Rabbit mAb (PBS Only)
db11649-PBS
Datasheet

Product Name : ERK1/2 (DGR11869) Rabbit mAb (PBS Only)

Cat.No.: db11649-PBS

Synonyms : ERT1; Erk2; ERK-2; p42-MAPK

Application：

Reactivity : Human, Mouse, Rat

Host species : Rabbit

Conjugation/Formulation：PBS Only

Un-conjugated PBS Only
  • Background kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]
  • Immunogen Recombinant protein of human ERK2
  • Gene ID 116590
  • Swiss Prot P28482
  • Synonyms ERT1; Erk2; ERK-2; p42-MAPK
  • Reactivity Human,Mouse,Rat
  • Application WB, ICC/IF, FC, IP
  • Calculated MW 43,41 kDa
  • Observed MW 44,42 kDa
  • Host species Rabbit
  • Clonality Monoclonal
  • Clonality No. DGR11869
  • Isotype IgG
  • Purity Affinity Purification
  • Conjugation Un-conjugated
  • Concentration 1 mg/mL
  • Formulation PBS Only
  • Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
×
  • Western blot analysis of extracts from K562, C6, 3T3, HeLa cells and Rat brain tissue using db11649 at 1:1000.
  • Immunofluorescent analysis of K562 cells using db11649 antibody (green), and DAPI (blue).
  • Immunofluorescence analysis of HeLa cells labelling ERK1/2 with db11649.

    The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11649 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.

    Control: Secondary antibody only.
订购信息
  • Package Price (RMB)
  • 10μg 2598
  • 100μg 7858
  • 货期：现货
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For research use only. Not intended for diagnostic.