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Survivin (DGR13233) Rabbit mAb (PBS Only)
db11658-PBS
Datasheet
Conjugation/Formulation：PBS Only
- Background This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
- Immunogen A synthetic peptide of human Survivin
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Gene ID
332
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Swiss Prot
O15392
- Synonyms API4; EPR-1
- Reactivity Human
- Application WB, IHC-P, ICC/IF, FC, IP
- Calculated MW 16 kDa
- Observed MW 16 kDa
- Host species Rabbit
- Clonality Monoclonal
- Clonality No. DGR13233
- Isotype IgG
- Purity Affinity Purification
- Conjugation Un-conjugated
- Concentration 1 mg/mL
- Formulation PBS Only
- Storage/Stability Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
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Western blot analysis of extracts from HeLa cells using db11658 at 1:1000. -
Immunofluorescence analysis of HeLa cells labelling Survivin with db11658.
The cells were fixed with 4% PFA (10min, RT) followed by treatment with 0.1% Triton X-100 (10min, RT), and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with db11658 (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (db10005, shown in green). Nuclear DNA was labeled in blue with DAPI.
Control: Secondary antibody only.
订购信息
- Package Price (RMB)
- 10μg 1498
- 100μg 4498
- 货期：现货
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For research use only. Not intended for diagnostic.
